Record-Breaking Lifespan Extension in Aged Rats Using Pig-Derived Exosomes: A Preclinical Study

TL;DR: Pig-derived exosomes (E5) significantly extended lifespan and improved health markers in female Sprague-Dawley rats, with one treated rat setting a world longevity record for its species at just over 4 years.

Key Findings:

  1. Lifespan Extension:
    • Control group: Oldest rat lived to 39.5 months; 50% died before 38 months
    • Treatment group: Earliest death at 38 months; 50% lived past 40 months
    • World record: One E5-treated rat lived slightly over 4 years
  2. Health Improvements: a. Strength: E5 doubled to tripled grip strength by day 150, maintaining superiority until study end b. Biomarkers:
    • Decreased: Blood urea nitrogen, TNF-α, IL-6, p53, NF-κB
    • Increased: GSH, SOD (antioxidants)
    • Unchanged: Weight, creatinine
  3. Methodology:
    • Subjects: 16 female Sprague-Dawley rats (8 per group)
    • Treatment: E5 administered 5 times (start, days 68, 181, 280, 377)
    • Duration: 420 days, starting at 24 months of age
  4. Potential Mechanisms:
    • Increased antioxidants
    • Decreased inflammation
    • Reduced NF-κB (associated with age-related diseases)
    • Delayed epigenetic alterations (hypothesized from decreased p53)
  5. Context:
    • Builds on previous research showing E5 reverses aging biomarkers in rats
    • Exosomes are cell-to-cell communication vesicles
    • EVs investigated for liver fibrosis treatment and as disease biomarkers
  6. Limitations and Future Directions:
    • Small sample size (16 rats total)
    • Exact mechanisms for record-breaking lifespan extension unclear
    • Further research needed to determine human applicability

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